Isotypes of autoantibodies against novel differential 4-hydroxy-2-nonenal-modified peptide adducts in serum is associated with rheumatoid arthritis in Taiwanese women

Table 1 Differential 4-hydroxy-2-nonenal (HNE)-modified peptide adducts in rheumatoid arthritis (RA) patients compared to healthy controls (HCs)

Protein^a	Modified peptide^b	Position^c	Obs.^d	Calc.^e	dM^f	dM^g	Sequence foundwith modifications
Protein^a	Modified peptide^b	Position^c	Obs.^d	Calc.^e	dM^f	dM^g	HC	RA
CFAH	SHTLRTTCWDGKLEYPTCAK(+ 158)	1211–1230	858.439	858.435	0.0041	5.4	−	+
CFAH	HRTGDEITYQCRNGFYPATRGNTAK(+ 138)	284–308	749.370	749.372	− 0.0018	−1.8	+	−
HPT	AVGDKLPECEADDGC(+ 158)PKPPEIAHGYVEHSVR	78–108	869.925	869.927	− 0.0017	−1.3	−	+
HPT	ILGGHLDA(+ 156)K	162–170	540.329	540.327	0.0028	6.1	+	−
IGKC	TVAA(+ 156)PSVFIFPPSDEQLK	2–19	701.384	701.386	− 0.0018	−1.7	−	+
	TVA(+ 156)APSVFIFPPSDEQLK	2–19	1051.582	1051.575	0.0070	7.1	+	+
	VYACEVTHQ(+ 158)GLSSPVTKSFNR	83–103	862.780	862.785	− 0.0044	−4.5	+	−
THRB	TFGSGEADCGLRPLFEK(+ 156)K	328–345	1056.036	1056.046	− 0.0107	−9.6	−	+
THRB	TFGSGEADC(+ 140)GLRPL(+ 156)FEK	328–344	708.376	708.375	0.0009	2.1	+	−

^aCFAH, Complement factor H; HPT, Haptoglobin; IGKC, Ig kappa chain C region; THRB, Prothrombin
^bSite of modified peptide
^cAmino acid positions of the first and the last residues in peptide
^dObs.: observed m/z of the modified peptides
^eCalc.: calculated m/z of the modified peptides
^fdM (Obs.-Calc.): mass accuracy
^gModified peptide mass accuracy (ppm)

ISSN: 1472-6947