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Fig. 2 | BMC Medical Informatics and Decision Making

Fig. 2

From: Isotypes of autoantibodies against novel differential 4-hydroxy-2-nonenal-modified peptide adducts in serum is associated with rheumatoid arthritis in Taiwanese women

Fig. 2

Protein levels of complement factor H (CFAH), haptoglobin (HPT), immunoglobulin kappa chain C region (IGKC), and prothrombin (THRB) in serum were respectively examined using anti-CFAH (a), anti-HPT (b), anti-IGKC (c), and anti-THRB (d) antibodies through Western blotting. Average densitometric values were calculated from duplicate data. Percentages of the SDS-PAGE gel and loading amounts of serum proteins used in Western blotting were 8% and 20 µg, 10% and 5 µg, 12% and 1 µg, and 8% and 20 µg for CFAH, HPT, IGKC, and THRB, respectively. Membrane Coomassie brilliant blue (CBB) staining was used as a loading control (bottom panel). Student’s t-test was used to determine the significance of blot densitometric differences, levels of serum proteins, and HNE-protein adducts. Values of mean ± 1 standard deviation are also indicated in the figure as horizontal lines

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