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Table 1 Semantic entities, applicable level (sentence, S or word(s), W), definitions and examples

From: Semantic text mining support for lignocellulose research

Semantic entity

Level

Definition

ActivityAssayConditions

S

Conditions at which the activity assay is carried out Ex.: disodium hydrogen phosphate, citric acid, pH 4.0, 37°C

Assay

W

Name of the activity assay Ex.: Dinitrosalicylic Acid Method (Somogyi-Nelson)

Enzyme

W

Enzyme name Ex.: alpha-galactosidase

Gene

W

Gene name Ex.: mel36F

Glycosylation

S

Presence of glycosylation on protein Ex.: N-glycosylated

Host

W

Organism used to produce the recombinant protein Ex.: Escherichia coli

KineticAssayConditions

S

Buffer, pH, temp. for the kinetic parameters determination Ex.: 0.1 M (disodium hydrogen phosphate, citric acid), pH 4.0, 37°C

Organism

W

Organism name Ex.: Gibberella sp.

pH

S

pH mentions Ex.: The enzyme retained greater than 90% of its original activity between pH 2.0 and 7.0 at room temperature for 3 h.

ProductAnalysis

S

Products formed from enzyme reaction and identification method Ex.: HPLC, glucose, galactose

SpecificActivity

S

Specific activity of the enzyme Ex.: 11.9 U/mg

Strain

W

Strain name Ex.: F75

Substrate

W

Substrate name Ex.: stachyose

SubstrateSpecificity

S

Substrate specificity mentions Ex.: The Endoglucanase from Pyrococcus furiosus had highest activity on cellopentaose

Temperature

S

Temperature mentions Ex.: The enzyme stability at different pH values was measured by the residual activity after the enzyme was incubated at 25°C for 3 h.

  1. The list of the semantic entities along with the level they apply (sentence or word level), their definition and an instance example is provided in Table 1.